The antiserum was produced against synthesized non-phosphopeptide derived from human ICAM-1 around the phosphorylation site of tyrosine 512 (K-K-Y<sup>P</sup>-R-L).
Western blot analysis of extracts from HUVEC cells, using ICAM-1(Ab-512) Antibody (OAEC00455, Lane 1, 2 and 3) and ICAM-1(Phospho-Tyr512) Antibody (OAEC00082, Lane 4, 5 and 6).