Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
形态:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Fluorescent confocal image of HeLa cells stained with PDK4 (C-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with OAAB16777 PDK4 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.
human skeletal muscle
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (Cat. #OAAB16777), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
human Adrenal tissue
Formalin-fixed and paraffin-embedded human Adrenal tissue reacted with PDK4 Antibody (C-term) (Cat. #OAAB16777), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
mouse skeletal muscle tissue
The PDK4 Antibody (C-term) (Cat# OAAB16777) is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.
K562 cell
PDK4 Antibody (C396) (Cat. #OAAB16777) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PDK4 antibody detected the PDK4 protein (arrow).