STAT3 Antibody - C-terminal region (Phospho-S727) (OAAB13539)
靶点:
STAT3
应用范围:
IF, WB
宿主:
Rabbit
适应物种:
Human
克隆性:
Polyclonal
保存条件:
Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
形态:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Fluorescent confocal image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with OAAB13539 STAT3 (C-term S727) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). Note the highly specific localization of the STAT3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).
Daudi cell
STAT3 Antibody (pS727) (Cat. #OAAB13539) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the STAT3 antibody detected the STAT3 protein (arrow).