HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human COPS3 with DYKDDDDK tag probed with OAEB02500 (1.0ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed with OAEB02500 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.