OAEB02396 (2ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
HEK293
HEK293 overexpressing CARD11 (RC222740) and probed with OAEB02396 (mock transfection in first lane), tested by Origene.
human Jurkats
OAEB02396 staining (1ug/ml) of human Jurkat cells (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.