NCBI Gene Id: 7157
Swissprot Id: P04637
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OKAG00349
HepG2 and MCF7 Nuclear Extract
p53 (Phospho-Ser20) DNA-Binding ELISA detects active p53 (Phospho-Ser20) in HepG2 and MCF7 Nuclear Extract. The HepG2 and MCF7 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.