Sandwich ELISA using OARD00017 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( Kappa+ Lambda) antibody OARD00020 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA Titer
Sandwich ELISA using OARD00017 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( Kappa+ Lambda) antibody OARD00020 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA Specificity
ELISA of human immunoglobulins shows OARD00017 reacted to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of OARD00017 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA Titer
A titer ELISA using OARD00017. The plate was coated with different amounts of human IgA. A serial dilution of OARD00017 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Human Lymph
Immunohistochemistry of human lymphoid tissue using anti-human IgA antibody OARD00017