ELISA of human immunoglobulins shows OARD00015 reacted to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of OARD00015 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA Titer
A titer ELISA using OARD00015. The plate was coated with different amounts of human IgG3Lambda. A serial dilution of OARD00015 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Human Tonsil
Immunohistochemistry of human tonsil using anti-lambda light chain antibody OARD00015