NCBI Gene Id: 6657
Swissprot Id: P48431
Purified Mouse Monoclonal Antibody (Mab) - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAB07021
NCI-H460 cell
Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
SY5Y cell
Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with OAAB07021 SOX2 primary antibody (1:100, 2 h at room temperature).
A549 cell
Fluorescent image of A549 cell stained with SOX2 Antibody(Cat#OAAB07021/SG110310AA).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).SOX2 immunoreactivity is localized to Nucleus significantly.
human lung carcinoma tissue
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody (Cat.#OAAB07021), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
SOX2
Western blot analysis of SOX2 Antibody (Cat.#OAAB07021) by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.
293 cell
Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat.#OAAB07021). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)