Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
形态:
Liquid. PBS with 0.09% (W/V) sodium azide.
亚型:
IgG1
规格:
400ul
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAB06612
E Coli
HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS monoclonal antibody (6AT18).
12tag protein
Western blot analysis of lysates from 12tag protein , This demonstrates the His tagged antibody detected the His tagged protein (arrow).
12 tag recombinant protein
Western blot analysis of lysate from 12 tag recombinant protein, using HIS Tag(Cat. #OAAB06612). OAAB06612 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.