Amount Used: 0.3 Ug/106 cells BALB/c splenocytes were incubated with rat IgG2a isotype, rat anti-mouse CD22 (2D6, rat IgG1), rat anti-mouse CD8 (53-6.7, rat IgG2a), rat anti-mouse CD45(LCA) (I3/2.3, rat IgG2b), rat anti-mouse CD24 (30-F1, rat IgG2c), and rat anti-mouse CD45RC (GL24, rat IgM). After washing, the cells were stained with FITC-labeled Mouse Anti-Rat IgG2b. Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).