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- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Sphingomyelin synthase-related protein 1 protein (1-152AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q96LT4
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
>95%,Protein G purified
- 靶点:
SAMD8
- 抗体英文名:
SAMD8 Antibody, HRP conjugated
- 抗体名:
SAMD8;Sphingomyelin synthase-related protein 1;SMSr;Ceramide phosphoethanolamine synthase;CPE synthase;Sterile alpha motif domain-containing protein 8;SAM domain-containing protein 8
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
功能
Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
单抗主要是鼠单抗、兔单抗,多抗主要是兔多抗、山羊多抗等,其他种属来源抗体较少。不建议纠结于单抗好还是多抗好,能做出实验的抗体就是好抗体。 在选择上一般建议实验种属和抗体种属亲缘性越远越好,不宜同源。抗体不同种属会要影响接下来二抗的选配。特别是在免疫荧光双标实验中,需要在区别于实验种属的基础上,区分开两个指标的抗体种属来源,以便于二抗区别识别。 3. 关于性价比的选择 抗体的品质高低最终还是需要通过实验结果来呈现的,但是在选择抗体之前我们并无法准确判断抗体的效果
once and for 5 minutes twice.4. Secondary antibody incubation: Incubate the blot in 1 % non-fat milk / PBST or Trident Universal Protein Blocking Reagent containing the HRP-conjugated secondary antibody at the proper dilution for one hour at RT.5. Washing: Wash the blot
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