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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Sphingomyelin phosphodiesterase 3 protein (401-655AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
FITC
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q9NY59
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
/
- 浓度:
>95%,Protein G purified
- 靶点:
SMPD3
- 抗体英文名:
SMPD3 Antibody, FITC conjugated
- 抗体名:
neutral membrane (neutral sphingomyelinase II) antibody
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
功能
Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验In vitro Sphingomyelinase Assay
= "cytosol" 9) Resuspend pellet in 3 ml lysis buffer. --> This fraction = "membrane" Preparing substrate 10) Resuspend dried 14C-SM in appropriate buffer to get 20 nmoles, 2 x 105 cpm, 100 µl per sample. Buffer A: For neutral, Mg-dependent
In vitro Sphingomyelinase Assay
In vitro Sphingomyelinase Assay Contributor: Suprya Jayadev Date: May 25, 1993 Reagents: Lysis buffer 25 mM Tris-HCl, pH 7.4 5 mM EDTA 1 mM ATP 20 µg/ml CLAP 1 mM PMSF Buffer A 10 mM MgCl2 0.2 M Tris
Southern Blot using Neutral Transfer
-Elaine Pinheiro and Aurora Burds Connor MIT, Jan2006 This protocol works very well with Hybond-XL membrane from GE Healthcare (formerly Amersham). 1) Digest your genomic DNA, using about 10 μg per reaction in 100 μl total digestion
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