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- 文献和实验
- 技术资料
- 免疫原:
Human MED19
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
/
- 标记物:
/
- 适应物种:
Human,Mouse,Rat
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
A0JLT2
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA,WB
- 浓度:
>95%,Antigen Affinity Purified
- 靶点:
MED19
- 抗体英文名:
MED19 Antibody
- 抗体名:
LCMR1 antibody;Lung cancer metastasis related protein 1 antibody;Lung cancer metastasis-related protein 1 antibody;med19 antibody;MED19_HUMAN antibody;Mediator complex subunit 19 antibody;Mediator of RNA polymerase II transcription subunit 19 antibody
- 规格:
100μl
保存缓冲液
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles.功能
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文献和实验whole-worm extracts. In principle, a protein-specific antibody is used to purify the protein of choice and its associated complex members from worm extract. This may also include RNA molecules associated with other protein components. To identify
96-well RNA In Situ Hybridization Protocol
to the bottom of the well.IV. BUFFER COMPOSITION AND VENDOR INFORMATION 2X Polymerase mix 287μlddH2 0 100μl10X Boehringer M. transcription buffer 50μl10X dig-NTP mix 13μlRNase inhibitor (500U) 50μlT3 or T7 RNA polymerase (1000U) 500μlTotal Volume 10X dig NTP mix
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
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