- ￥880 - 1320
- CUSABIO
- CN
- CSB-PA000540
- 2025年07月14日
- WB, IHC, ELISA; WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:10000
- Rabbit
- Human,Mouse,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from Human Histone H2A.X around the phosphorylation site of S139.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性：
/
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse,Rat
- 保质期：
6个月
- 抗原来源：
Homo sapiens (Human)
- 目录编号：
P16104
- 级别：
优
- 库存：
200
- 供应商：
武汉华美生物工程有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA; WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:10000
- 浓度：
>95%,The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 靶点：
H2AFX
- 抗体英文名：
Phospho-Histone H2A.X (S139) Antibody
- 抗体名：
H2A histone family member X antibody;H2A histone family member X antibody;H2A.FX antibody;H2A.X antibody;H2a/x antibody;H2AFX antibody;H2AX antibody;H2AX_HUMAN antibody;Histone H2A.X antibody
- 规格：
100μg/50μg
| 规格： | 100μg | 产品价格： | ￥1320.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥880.0 |
保存缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.功能
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文献和实验cells at 3000 X g. Resuspend in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition of beta
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
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