相关产品推荐更多 >

IER3抗体IER3兔多抗抗体Protein PRG1抗体IER3 Antibody抗体
¥440
RAB35抗体RAB35一抗抗体RAY antibody抗体RAB35 Antibody抗体
¥880
TRBC2抗体TRBC2一抗抗体TCRBC2抗体TRBC2 Antibody抗体
¥440
HIST1H3A抗体HIST1H3A兔多抗抗体Histone H3/l antibody抗体Tri-methyl-HIST1H3A (K36) Antibody抗体
¥660
RAB12抗体RAB12一抗抗体Ras-related protein Rab-12 antibody抗体RAB12 Antibody抗体
¥3900
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from the Internal region of Human GPR149.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
/
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q86SP6
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF, ELISA; WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:5000
- 浓度:
>95%,The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 靶点:
GPR149
- 抗体英文名:
GPR149 Antibody
- 抗体名:
GPR149;PGR10;Probable G-protein coupled receptor 149;G-protein coupled receptor PGR10
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
保存缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.功能
/风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization
Immunocytochemistry exploits the incomparable specificity of the antibody-antigen interaction to form the basis of a flexible approach to the study of expression and localization of proteins both in model systems and their physiological context
少可以参考文献上给定的硫酸铵固体的溶解度表,《Guide to Protein Purification》一书中就有详细的溶解度表,静置两个小时左右,10000 g离心半个小时后,弃去上清,用PBS重新溶解沉淀,然后再次加入硫酸铵固体至45%饱和,静置两个小时左右10000 g离心半个小时,弃去上清,用少量PBS溶解沉淀,即得到较纯的抗体,因为此抗体中尚有较高浓度的硫酸铵,因此需要对PBS透析,一般透析一天一夜较合适,透析过程中更换透析液两至三次。如果对纯度要求不是很高,也可以不做第一次沉淀,直接用45
.7. Wang, X. et al. 2011. Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5. Sci Rep1: 102 (DOI:10.1038/srep00102).8. Wang, X. and Zhang, S. 2011. Production of a bioengineered G-protein coupled receptor of human
技术资料暂无技术资料 索取技术资料





