- ￥880 - 1320
- CUSABIO
- CN
- CSB-PA009006
- 2025年07月15日
- IHC, ELISA; IHC:1:100-1:300, ELISA:1:5000
- Rabbit
- Human,Mouse,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from Human GRF-1 around the non-phosphorylation site of Y1105.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性：
/
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse,Rat
- 保质期：
6个月
- 抗原来源：
Homo sapiens (Human)
- 目录编号：
Q9NRY4
- 级别：
优
- 库存：
200
- 供应商：
武汉华美生物工程有限公司
- 宿主：
Rabbit
- 应用范围：
IHC, ELISA; IHC:1:100-1:300, ELISA:1:5000
- 浓度：
>95%,The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 靶点：
ARHGAP35
- 抗体英文名：
ARHGAP35 Antibody
- 抗体名：
Rho GTPase-activating protein 35 antibody
- 规格：
100μg/50μg
| 规格： | 100μg | 产品价格： | ￥1320.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥880.0 |
保存缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.功能
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文献和实验35 Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells
The technologies described in this volume enable the isolation of recombi- nant antibodies (Abs) from libraries of variable regions (Fvs) or Fabs displayed on the surface of filamentous phage by fusion to a structural protein
Metabolic Labeling of Cells with 35S
1) Transfer to a 24 wells plate the desired colonies. 2) Once the cells are attached (at least 8 hours after tripsinizing them) add ~1 ml of DME (met-, cys-). 3) Add 3l of Met 35S, Cys 35S trans label. 4) Incubate at least 5 hours at 370C
protein-protein interactions with in vitro transcribed and translated proteins. The two proteins should be translated such that one is labelled with 35S methionine and one is unlabelled. If binding is to be assayed by Immunoprecipitation
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