- ￥3390.30
- Merck millipore
- MABE419
- 2025年07月13日
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文献和实验Polyclonal and Monoclonal Antibodies
. Do not disturb the cells. g) Spin at 1000 rpm for 5 min. h) Aspirate medium, resuspend cells by tapping. Slowly add 5 ml of 37 C IMDM-m. i) Bring to 20 ml and add to the flask in the incubator. (If using feeder cells, add them at this point; 106
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Western Blotting with Monoclonal Ant
the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies). 3.Decant the wash buffer, add the diluted enzyme conjugate and incubate with agitation for 30 minutes
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