手机验证
-20℃
长期
pGL6-TA Reporter gene plasmid
856
北京百奥莱博科技有限公司
1μg
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博主要从事生物试剂的生产和销售,产品线涵盖北京现货pGL6-TA报告基因质粒怎么卖在内的分子生物学试剂产品,还包括PCR及荧光定量PCR、核酸提取试剂盒类、样本核酸保护试剂、核酸提取、克隆与转化、蛋白纯化、蛋白检测、免疫组化、细胞凋亡与抗体等。
名称:北京现货pGL6-TA报告基因质粒怎么卖
产地:国产|进口
规格:1μg
品牌:百奥莱博
pGL6-TA报告基因质粒是用于在哺乳动物细胞中进行萤火虫萤光素酶报告基因检测的新一代质粒。该报告基因质粒比Promega公司的pGL3系列有了全面的改进,一方面对于luciferase的编码进行了改进,确保能更好地在哺乳动物细胞中进行表达,同时对整个质粒中所有可以被预测出的可能的转录因子结合位点全部进行了适当的突变处理,在保持原有功能不变的情况下,使各种转录因子在质粒上的非特异性结合降到最低。
pGL6-TA主要用于在其多克隆位点插入特定的启动子、增强子等调控元件研究该调控序列的基因转录调控活性。pGL6-TA和pGL6相比在多克隆位点和luc基因之间加入了一段minimal TA promoter,使luc基因的基础转录水平提高。
pGL6-TA质粒的主要信息如下:
Base pairs | 5629 |
Multiple cloning region | 1-59 |
Minimal TA promoter (pTA) | 66-88 |
luc2 reporter gene | 120-1782 |
SV40 late poly(A) signal | 1817-2038 |
SV40 early enhancer/promoter | 2086-2504 |
Synthetic neomycin phosphotransferase(Neor)coding region | 2529-3323 |
Synthetic poly(A) signal | 3348-3396 |
Reporter Vector primer 4 (RVprimer4) binding region | 3463-3482 |
ColE1-derived plasmid replication origin | 3720 |
Synthetic Beta-lactamase (Ampr) coding region | 4511-5371 |
Synthetic poly(A) signal/transcriptional pause site | 5476-5629 |
Reporter Vector primer 3 (RVprimer3) binding region | 5578-5597 |
About this Antibody | |
Name | TOP2A Rabbit Polyclonal Antibody |
Category | Polyclonal antibody(pAb); Primary antibody |
Isotype | IgG |
Purification | Peptide affinity purified |
About the Immunogen | |
Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to TOP2A. |
Gene ID | 7153 |
SwissProt | P11388 |
Synonyms | DNA topoisomerase II; DNA topoisomerase II-alpha; EC 5.99.1.3, P11388-1; TOP-2; TOP2; TOP2A; TP2A; alpha isozyme |
Category | Chromatin Regulation / Nuclear Function |
Background | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. It is essential during mitosis and meiosis for proper segregation of daughter chromosomes. |
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