Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of G1049S, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes G1049S mutant, but not wild-type PIK3CA of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Western blot:
Western blot analysis of recombinant PIK3CA (G1049S) and wildtype proteins. Purified His-tagged PIK3CA (G1049S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-PIK3CA (G1049S) monoclonal antibody (Cat. #26219).