Background: EML4-ALK is a fusion-type protein kinase that can be found in many tumors, such as anaplastic large cell lymphoma, inflammatory myofibroblastic tumor, neuroblastoma and NSCLC, and so on. It is generated as a result of a small inversion within the short arm of human chromosome 2. EML4-ALK protein often promotes and maintains the malignant behavior of the cancer cells by activating the MAPK, PI3K/AKT and JAK/STAT3 pathways.
Immunogen: A synthetic peptide from the internal region of EML4-ALK of human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes T41A mutant, but not wild-type EML4-ALK of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant EML4-ALK and wildtype proteins. Purified His-tagged EML4-ALK protein (lane 3), corresponding wild-type EML4 protein (lane1) and wild-type ALK protein (lane2) were blotted with anti-EML4-ALK monoclonal antibody (Cat. #26242).
Immunofluorescence:
Immunofluorescence of cells expressing EML4-ALK proteins with anti-EML4-ALK antibody. HEK293T cells were transfected with pCDNA3-GFP-EML4 (WT) plasmid (left column) or pCDNA3-GFP-ALK (WT) plasmid (middle column) or pCDNA3-GFP-EML4-ALK plasmid (right column), then fixed and stained with anti-EML4-ALK monoclonal antibody (Cat. #26242).