Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of D761Y, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes EGFR (D761Y), but not wild type EGFR from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
Western blot:
Western blot analysis of recombinant EGFR (D761Y) and wildtype proteins. Purified His-tagged EGFR (D761Y) protein (amino acids 702-987, lane 2, Cat. # 10240) and corresponding wild type protein (lane 1, Cat. #10210) were blotted with anti-EGFR (D761Y) mouse monoclonal antibody (Cat. #26120). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (D761Y) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (D761Y) plasmid (right column), then fixed and stained with anti-EGFR (D761Y) monoclonal antibody (Cat. #26120).