- ￥980 - 4800
- Bioss
- bsm-0295M
- 2025年10月10日
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￥980
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1mg/10mg
| 规格： | 1mg | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥4800.0 |
| 产品编号 | bsm-0295M |
| 英文名称 | Mouse Anti-Rabbit IgG mAb |
| 中文名称 | 小鼠抗兔IgG单克隆抗体 |
| 英文别名 | Rabbit Immunoglobulin G; Rb IgG; |
| 产品应用 | WB=1:5000-50000, IHC-P=1:100-500, IHC-F=1:1000-5000, ICC/IF=1:1000-5000, IF=1:1000-5000, ELISA=1:5000-50000 Not yet tested in other applications. |
| 交叉反应 | Rabbit |
| 抗体来源 | Mouse |
| 免疫原 | Native Rabbit IgG |
| 亚型 | IgG1 |
| 性状 | Lyophilized |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:5000-50000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:1000-5000} |
| {ICC/IF} | {1:1000-5000} |
| {IF} | {1:1000-5000} |
| {ELISA} | {1:5000-50000} |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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