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- bs-0309R-HRP
- 2025年10月10日
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- 规格：
100ul/1ml
| 规格： | 100ul | 产品价格： | ￥180.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥1200.0 |
| 产品编号 | bs-0309R-HRP |
| 英文名称 | Rabbit Anti-Pig IgG H&L, HRP conjugated |
| 中文名称 | 辣根过氧化物酶标记的兔抗猪IgG H&L |
| 英文别名 | Rabbit Anti-Pig IgG H&L (HRP); Immunoglobulin G; |
| 产品应用 | WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-1000, ELISA=1:1000-10000 Not yet tested in other applications. |
| 交叉反应 | Pig |
| 抗体来源 | Rabbit |
| 免疫原 | Native Pig IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-1000} |
| {ELISA} | {1:1000-10000} |
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文献和实验[IF={{ 7.78 }}] {Wei, Bo, et al. "Magnetic beads-based enzymatic spectrofluorometric assay for rapid and sensitive detection of antibody against ApxIVA of Actinobacillus pleuropneumoniae." Biosensors and Bioelectronics 35.1 (2012): 390-393.} {Other} {="Pig"}
[IF={{ 3.471 }}] {Lijun Zhang. et al. A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs. FRONT VET SCI. 2022; 9: 902497} {ELISA} {Mouse, Pig}
[IF={{ 3.471 }}] {Tongsheng Qi. et al. Seroepidemiology of Neosporosis in Various Animals in the Qinghai-Tibetan Plateau. FRONT VET SCI. 2022 Jul 19;9:953380} {ELISA} {Neospora caninum}
[IF={{ 3.43 }}] {Li, XuePu, et al. "Sensitive immunoassay for porcine pseudorabies antibody based on fluorescence signal amplification induced by cation exchange in CdSe nanocrystals." Microchimica Acta 180.3-4 (2013): 303-310.} {ELISA} {="Pig"}
[IF={{ 3.412 }}] {Peng C et al. Soybean Glycinin- and β-Conglycinin-Induced Intestinal Damage in Piglets via the p38/JNK/NF-κB Signaling Pathway. J Agric Food Chem. 2018 Sep 12;66(36):9534-9541.} {WB} {Pig}
人抗风疹病毒IgG抗体（anti-RV IgG）酶联免疫分析（ELISA）
中依次加入抗风疹病毒 IgG 抗体（ anti-RV IgG ），再与 HRP 标记的抗原结合，形成抗原 - 抗体 - 酶标抗原复合物，经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体（ anti-RV IgG ）呈正相关。用酶标仪在 450nm 波长下测定吸光度（ OD 值），通过标准曲线计算样品中人抗风疹病毒 IgG 抗体（ anti-RV IgG ）浓度。 试剂盒组成
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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