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- bsF-0362P
- 2025年10月16日
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300ul
| 产品编号 | bsF-0362P |
| 英文名称 | Protein A / FITC |
| 中文名称 | FITC标记重组金*色葡***蛋白A |
| 英文别名 | SPA; Recombinant SPA; Protein A / FITC |
| 性状 | Liquid |
| 浓度 | 1mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > Protein A |
| 亚细胞定位 | Secreted; cell wall. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies. |
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文献和实验Microsphere Surface Protein Determination Using Flow Cytometry
., and Price, N.C. 1999. Colorimetric protein assay techniques. Biotechnol. Appl. Biochem. 29:99‐108. Sklar, L.A., Edwards, B.S., Graves, S.W., Nolan, J.P., and Prossnitz, E.R. 2002
CGH Image acquisition Images were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeiss, Oberkochen, Germany) equipped with filter sets appropriate for DAPI (Zeiss filter set
A PC-based program for evaluation of Comparative Genomic Hybridization (CGH) experiments
FITC and TRITC images at the position x,y. For all chromosome pixels a FITC/TRITC ratio image r(x,y) is calculated as: r(x,y) := ( f(x,y) * 128 * Mt ) / ( t(x,y) * Mf ) for f(x,y) >= t(x,y) r(x,y) := 256 - (( t(x,y) * 128 * Mf ) / ( f(x,y
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