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- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-17419R |
| 英文名称 | Histone H1.4 (tri methyl K25, phospho S26) Rabbit pAb |
| 中文名称 | 磷酸化三甲基化组蛋白H1抗体 |
| 英文别名 | H1 histone family member 4; H1.4; H14_HUMAN; H1E; H1F4; Hist1h1e; Histone 1 H1e; Histone cluster 1 H1e; Histone H1; Histone H1.4; Histone H1b; MGC116819. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Dog, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Histone H1.4 around the site of tri methyl-Lys25+phospho-Ser26 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 22 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Histones > H1 > Unmodified Epigenetics and Nuclear Signaling > Histones > Variants |
| 亚细胞定位 | Nucleus. Chromosome. |
| 翻译后修饰 | Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. |
| 相似性 | Belongs to the histone H1/H5 family. Contains 1 H15 (linker histone H1/H5 globular) domain. |
| 功能 | Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Histone H1b are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验Isolation of CpG Islands Using a Methyl-CpG Binding Column
. 1 and 2 for reviews). In addition to distinctive DNA characteristics, CpG islands also have an open chromatin structure in that they are hyperacetylated, lack histone H1, and have a nucleosome-free region (3) . The major reason for interest in CpG islands
Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column
. 1 and 2 for reviews). In addition to distinctive DNA characteristics, CpG islands have an open chromatin structure in that they are hyperacetylated, lack histone H1, and have a nucleosome-free region (3 ). The major reason for interest in CpG islands
) or presence (+) of okadaic acid (500 nM; Calbiochem), an inhibitor of cellular phosphatases. Acid‐soluble nuclear proteins and isolated histone H1 were subjected to electrophoresis in a TAU gel and analyzed by Coomassie blue staining. Lane M contains
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