Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-IRGC Polyclonal Antibody, Unconjugated(bs-16706R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-16706R
- 2026年10月14日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-16706R |
| 英文名称 | IRGC Rabbit pAb |
| 中文名称 | IRGC蛋白抗体 |
| 英文别名 | F630044M05Rik; Gm1102; Gm474; IIGP5; IIGP5_HUMAN; Immunity-related GTPase cinema 1; Interferon-inducible GTPase 5; IRGC; IRGC1; RGD1311107. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IRGC |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 相似性 | Belongs to the interferon-inducible GTPase family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IRGC (Immunity Related GTPase Cinema) is a Protein Coding gene. Diseases associated with IRGC include Solar Retinopathy and Echolalia. GO annotations related to this gene include GTP binding and hydrolase activity, acting on acid anhydrides. An important paralog of this gene is IRGM. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
_x000D_ A549 Cell (Human) Lysate at 30 ug
_x000D_ Primary: Anti-IRGC (bs-16706R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kD
_x000D_ Observed band size: 50 kD
_x000D_ A549 Cell (Human) Lysate at 30 ug
_x000D_ Primary: Anti-IRGC (bs-16706R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kD
_x000D_ Observed band size: 50 kD
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IRGC Rabbit pAb(bs-16706R)-50ul/100ul/200ul
￥1180 - 2800
