Protein: K562(human) lysates at 30ug;
Primary: rabbit Anti-DNA primase (bs-14357R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:60 kD
Observed band size:60 kD
- ￥1180 - 2800
- Bioss
- bs-14357R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-14357R |
| 英文名称 | DNA Primase Rabbit pAb |
| 中文名称 | DNA引物酶抗体 |
| 英文别名 | dJ422B11.1.1; DNA primase 58kDa subunit; DNA primase large subunit; DNA primase p58; DNA primase subunit p58; p58; Polypeptide 2A p58; PRIM2; PRIM2A; PRI2_HUMAN; Primase DNA polypeptide 2(58kDa); Primase polpeptide 2A(58kD); Primase polpeptide 2A 58kDa. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Pig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human DNA Primase |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 59 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding Epigenetics and Nuclear Signaling > DNA / RNA > DNA Synthesis |
| 亚基 | Heterodimer of a small subunit and a large subunit. |
| 相似性 | Belongs to the eukaryotic-type primase large subunit family. |
| 功能 | DNA primase synthesizes small ribonucleotide primers for the initiation of DNA replication. The eukaryotic primase is a heterodimer of a small and a large subunit, p49 and p58 respectively. These two subunits purify as a complex tightly bound to DNA polymerase alpha. This tight association implicates polymerase alpha as the lagging-strand DNA polymerase in replication. Uniquely, primases are able to synthesize nucleotides de novo on a template by the formation of an initial dinucleotide. DNA primase initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primer template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of DNA primase contains the catalytic active site. The human primase subunits are 90% identical in amino acid sequence to the mouse homologs. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. [provided by RefSeq, Sep 2013] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
bone (mouse) Lysate at 40 ug
testis (Mouse) Lysate at 40 ug
Primary: Anti-DNA primase (bs-14357R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 63 kD
bone (mouse) Lysate at 40 ug
testis (Mouse) Lysate at 40 ug
Primary: Anti-DNA primase (bs-14357R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 63 kD
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文献和实验该产品被引用文献
[IF={{ 1.7 }}] {Yin Jingyang. et al. Increased PRIM2 expression associated with poor-prognosis in patients with pancreatic ductal adenocarcinoma. PANCREAS. 2024 Sep;:10.1097/MPA.0000000000002387} {IHC} {Human}
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DNA Primase Rabbit pAb(bs-14357R)-50ul/100ul/200ul
￥1180 - 2800
