Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AP1M2 Polyclonal Antibody, Unconjugated(bs-13638R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-13638R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13638R |
| 英文名称 | AP1M2 Rabbit pAb |
| 中文名称 | AP-1μ2抗体 |
| 英文别名 | Adaptor protein complex AP-1 mu-2 subunit; Adaptor protein complex AP1 mu2 subunit; Adaptor related protein complex 1 mu2 subunit; Adaptor-related protein complex 1 mu-2 subunit; AP mu chain family member mu1B; AP-1 complex subunit mu-2; AP-mu chain family member mu1B; AP1 complex subunit mu2; AP1M2; AP1M2_HUMAN; AP47 2; Clathrin assembly protein complex 1 medium chain 2; Golgi adaptor HA1/AP1 adaptin mu-2 subunit; Golgi adaptor HA1/AP1 adaptin mu2 subunit; HA1 47 kDa subunit 2; HSMU1B; MU 1B; Mu adaptin 2; Mu-adaptin 2; Mu1B adaptin; Mu1B-adaptin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human AP1M2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Adapters > Cytoplasmic Signal Transduction > Protein Trafficking > Vesicle Transport > Adapters |
| 亚细胞定位 | Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. |
| 翻译后修饰 | Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals. |
| 相似性 | Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human A431 cell lysates
Primary: Anti-AP1M2 (bs-13638R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human A431 cell lysates
Primary: Anti-AP1M2 (bs-13638R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa
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AP1M2 Rabbit pAb(bs-13638R)-50ul/100ul/200ul
￥1180 - 2800
