Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Laminin 5 Polyclonal Antibody, Unconjugated(bs-6713R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-6713R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6713R |
| 英文名称 | Laminin 5 Rabbit pAb |
| 中文名称 | 层粘连蛋白5抗体 |
| 英文别名 | E170; Epiligrin 170 kDa subunit; Epiligrin subunit alpha; Kalinin subunit alpha; LAMA3; LAMA3A; Laminin 5 alpha3; Laminin A3; laminin alpha 3; laminin alpha3; Laminin subunit alpha-3; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; laminin5 alpha 3; Laminin5 alpha3; LAMNA; LOCS; Nicein subunit alpha; LAMA3_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LAMA3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 367 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Extracellular matrix Cardiovascular > Angiogenesis > Adhesion / ECM > Extracellular Matrix Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Intermediate Filaments Neuroscience > Cell Adhesion Proteins > ECM Proteins Signal Transduction > Cytoskeleton / ECM > Basal Lamina |
| 亚基 | Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-3 is a subunit of laminin-5 (laminin-332 or epiligrin/kalinin/nicein), laminin-6 (laminin-311 or K-laminin) and laminin-7 (laminin-321 or KS-laminin). |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. |
| 组织特异性 | Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS. |
| 相似性 | Contains 15 laminin EGF-like domains. Contains 5 laminin G-like domains. Contains 1 laminin IV type A domain. Contains 1 laminin N-terminal domain. |
| 功能 | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin 5 is an isoform composed of three distinct subunits, alpha 3, beta 3 and gamma 2, which are bound to each other in a cross-shaped molecule by disulphide bonds. It is a complex glycoprotein thought to be involved in cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes. It is also involved in signal transduction via tyrosine phosphorylation of pp125-FAK and p80, and differentiation of keratinocytes. The laminin alpha 3 subunit is also thought to be a component of laminin 6 and laminin 7 |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 9.7 }}] {Inoue et al. Exercise restores muscle stem cell mobilization, regenerative capacity and muscle metabolic alterations via adiponectin/AdipoR1 activation in SAMP10 mice. (2017) J.Cachexia.Sarcopenia.Muscle. 8:370-385} {IF} {Mouse}
[IF={{ 8.079 }}] {Piao, Limei. et al. Human umbilical cord-derived mesenchymal stromal cells ameliorate aging-associated skeletal muscle atrophy and dysfunction by modulating apoptosis and mitochondrial damage in SAMP10 mice. STEM CELL RES THER. 2022 Dec;13(1):1-17} {IF} {Mouse}
[IF={{ 7.7 }}] {Li Wang. et al.Phase-transited lysozyme coating on zirconia abutments for enhancing soft tissue seal and antibacterial activity..International Journal of Biological Macromolecules.2025 Mar 12;307(Pt 3):142016.} {IF} {Human}
[IF={{ 12.063 }}] {Aiko Inoue. et al. Young bone marrow transplantation prevents aging-related muscle atrophy in a senescence-accelerated mouse prone 10 model. J CACHEXIA SARCOPENI. 2022 Sep;:} {IF} {Mouse}
[IF={{ 10.02 }}] {Jiang, Haiying, et al. "Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia." Nature Communications 5 (2014).} {WB} {="Rat"}
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Laminin 5 Rabbit pAb(bs-6713R)-50ul/100ul/200ul
￥1180 - 2800
