Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IRX4 Polyclonal Antibody, Unconjugated(bs-9464R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-9464R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9464R |
| 英文名称 | IRX4 Rabbit pAb |
| 中文名称 | Iroquois同源蛋白4抗体 |
| 英文别名 | Homeodomain protein IRXA3; Iroquois Homeobox Protein 4; Iroquois-class homeodomain protein IRX-4; Iroquoisclass homeodomain protein IRX4; Irx4; IRX4_HUMAN; IRXA3. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IRX4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 54 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Heart > Cardiogenesis > Transcription factors/regulators Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families > IRX/IRO Epigenetics and Nuclear Signaling > Transcription > Transcription Factors |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Predominantly expressed in cardiac ventricles. |
| 相似性 | Belongs to the TALE/IRO homeobox family. Contains 1 homeobox DNA-binding domain. |
| 功能 | Likely to be an important mediator of ventricular differentiation during cardiac development. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The Iroquois homeobox gene family of transcription factors regulate aspects of embryonic development including anterior/posterior and dorsal/ventral axis patterning in the central nervous system. The Iroquois family are clustered on two loci, IRXA and IRXB, which map to chromosomes 8 and 13 in mice. The IRXA group includes Irx1, Irx2 and Irx4; the IRXB group is comprised of Irx3, Irx5 and Irx6. Irx1 and Irx2 are both widely expressed during development in the lung epithelium and also in the ventricular septum. Irx1 and Irx2 also play a role in digit formation (E11.5–E14.5). The Irx gene family members are each expressed in a distinct pattern during mouse heart development. Specifically, Irx1 and Irx2 are expressed in the ventricular septum and Irx3 is expressed in the ventricular trabeculated myocardium. In addition, Irx4 is expressed in the linear heart tube and the AV canal, and Irx5 is expressed in the endocardium lining the ventricular and atrial myocardium. Furthermore, the IRX4 gene may modulate cardiac development and function. Although the heart of Irx4(-) mice appears to develop normally, adult Irx4(-) mice exhibit cardiomyopathy, including cardiac hypertrophy and decreased contractility. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Brain (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-IRX4 (bs-9464R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 54 kD
Observed band size: 57 kD
Heart (Mouse) Lysate at 40 ug
Primary: Anti-IRX4 (bs-9464R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 54 kD
Observed band size: 57 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- IRX4 (bs-9464R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 52 kD
Heart (Mouse) Lysate at 40 ug
Primary: Anti- IRX4 (bs-9464R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 52 kD
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[IF={{ 1.5 }}] {Yuan, C.-W. et al. Incomplete radiofrequency ablation promotes the development of CD133+ cancer stem cells in hepatocellular carcinoma cell line HepG2 via inducing SOX9 expression. (2018) Hepatobiliary & Pancreatic Diseases International. S1499-3872(18)30202-9.} {FCM} {human}
[IF={{ 1.19 }}] {Bhattacharya, Subarna, et al. "High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry." JoVE (Journal of Visualized Experiments) 91 (2014): e52010-e52010.} {Other} {=""}
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IRX4 Rabbit pAb(bs-9464R)-50ul/100ul/200ul
￥1180 - 2800
