Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PMPCB Polyclonal Antibody, Unconjugated(bs-7003R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-7003R
- 2025年10月16日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7003R |
| 英文名称 | PMPCB Rabbit pAb |
| 中文名称 | 线粒体导肽水解酶β抗体 |
| 英文别名 | beta MPP; betaMPP; Mitochondrial processing peptidase subunit beta; MPP11; MPPB; MPPP52; P 52; P52; Peptidase mitichondrial processing beta. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PMPCB |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 49 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers > Tumor antigens Cancer > Tumor immunology > Tumor-associated antigens Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Tags & Cell Markers > Subcellular Markers > Organelles > Mitochondria |
| 亚基 | Heterodimer of alpha and beta subunits. |
| 亚细胞定位 | Mitochondrion matrix. |
| 相似性 | Belongs to the peptidase M16 family. |
| 功能 | Cleaves presequences (transit peptides) from mitochondrial protein precursors. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | PMPCB is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. PMPCB is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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PMPCB Rabbit pAb(bs-7003R)-50ul/100ul/200ul
￥1180 - 2800
