Sample:
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Lane 1: Mouse Liver tissue lysates
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Lane 2: Rat Kidney tissue lysates
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Primary: Anti-GPX2 (bs-13396R) at 1/1000 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 22 kDa
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Observed band size: 22 kDa
- ￥1180 - 2800
- Bioss
- bs-13396R
- 2026年10月14日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13396R |
| 英文名称 | GPX2 Rabbit pAb |
| 中文名称 | 谷胱甘肽过氧化酶2抗体 |
| 英文别名 | Gastrointestinal glutathione peroxidase; GI GPx; Glutathione peroxidase 2(gastrointestinal); Glutathione peroxidase 2; Glutathione peroxidase gastrointestinal; Glutathione peroxidase related protein 2; Glutathione peroxidase-gastrointestinal; Glutathione peroxidase-related protein 2; GPRP; GPRP-2; GPx 2; GPx-2; GPx-GI; GPX2; GPX2_HUMAN; GSHPx 2; GSHPx GI; GSHPx-2; GSHPx-GI. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GPX2/Glutathione Peroxidase 2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 22 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Redox metabolism > Antioxidants Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress |
| 亚基 | Homotetramer. |
| 亚细胞定位 | Cytoplasm. Mainly cytoplasmic. |
| 组织特异性 | Mostly in liver and gastrointestinal tract, not found in heart or kidney. |
| 相似性 | Belongs to the glutathione peroxidase family. |
| 功能 | Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1–8). GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GPX2) Polyclonal Antibody, Unconjugated (bs-13396R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF=64.8] Lorenzo-Martín L. Francisco. et al. Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo. NATURE. 2024 Apr;:1-8 IF Mouse
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GPX2 | GPx-GI Rabbit pAb(bs-13396R)-50ul/100ul/200ul
￥1180 - 2800
