Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human THP-1 cell lysates
Primary: Anti-NSF (bs-11255R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 75 kDa
- ￥1180 - 2800
- Bioss
- 北京
- bs-11255R
- 2025年10月15日
- WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated synthetic peptide derived from human NSF
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 保存条件：
Store at -20 °C for one year.
- 克隆性：
单克隆
- 标记物：
无
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,
- 保质期：
1年
- 目录编号：
bs-11255R
- 库存：
999
- 供应商：
北京博奥森生物技术有限公司
- 宿主：
Rabbit
- 应用范围：
WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ICC=1:100-500,
- 浓度：
1mg/ml
- 抗体英文名：
NSF
- 抗体名：
N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.00 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.00 |
| 规格： | 200ul | 产品价格： | ￥2800.00 |
| 产品编号 | bs-11255R |
| 英文名称 | NSF Rabbit pAb |
| 中文名称 | N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体 |
| 英文别名 | N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF; NSF_HUMAN; SKD 2; SKD2; SKD2; Vesicle fusing ATPase; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular fusion protein NSF; Vesicular-fusion protein NSF. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NSF |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 83 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16 (By similarity). |
| 亚细胞定位 | Cytoplasm. |
| 翻译后修饰 | Phosphorylation at Ser-569 interferes with homohexamerization (By similarity). |
| 相似性 | Belongs to the AAA ATPase family. |
| 功能 | Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4). |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
U937 (Human) Cell Lysate at 30 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-NSF (bs-11255R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kD
Observed band size: 83 kD
U937 (Human) Cell Lysate at 30 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-NSF (bs-11255R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kD
Observed band size: 83 kD
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anti-NSF
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