Sample:Testis (Mouse) Lysate at 40 ug
Primary: Anti-RAD50(bs-11230R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 154kD
Observed band size: 154kD
- ￥1180 - 2800
- Bioss
- bs-11230R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11230R |
| 英文名称 | RAD50 Rabbit pAb |
| 中文名称 | DNA修复蛋白Rad50抗体 |
| 英文别名 | DNA repair protein RAD50; hRad50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog(S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50_HUMAN; Rad50l; Truncated RAD50 protein. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=0.2μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human RAD50 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 154 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromosome Structure > Telomeres Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > Homologous Recomb. Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > Non Homol. End Joining |
| 亚基 | Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. |
| 亚细胞定位 | Nucleus. Chromosome telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents. |
| 组织特异性 | Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR |
| 相似性 | Belongs to the SMC family. RAD50 subfamily.
Contains 1 zinc-hook domain. |
| 功能 | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {0.2μg/Test} |
Blank control: 293T cells(fixed with 2% Paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-RAD50 antibody(bs-11230R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Primary Antibody:Rabbit Anti-RAD50 antibody(bs-11230R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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[IF={{ 5.572 }}] {Yue Zhang. et al. Dietary selenium excess affected spermatogenesis via DNA damage and telomere-related cell senescence and apoptosis in mice. FOOD CHEM TOXICOL. 2023 Jan;171:113556} {WB} {Mouse}
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RAD50 Rabbit pAb(bs-11230R)-50ul/100ul/200ul
￥1180 - 2800
