Blank control(blue): Hela(fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-KinesinKIF5B/FITC Conjugated antibody (bs-11026R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
- ￥1180 - 2800
- Bioss
- bs-11026R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11026R |
| 英文名称 | Kinesin/KIF5B Rabbit pAb |
| 中文名称 | 驱动蛋白KNS1抗体 |
| 英文别名 | Conventional kinesin heavy chain; KIF5B; Kinesin 1(110-120kD); Kinesin family member 5B; Kinesin heavy chain; Kinesin heavy chain ubiquitous; Kinesin-1 heavy chain; KINH; KINH_HUMAN; KNS 1; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC. |
| 产品应用 | Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Kinesin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 106 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Motor Proteins > Kinesin |
| 亚基 | Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and PPP1R42 (By similarity). Interacts with SYBU. Interacts with JAKMIP1. Interacts with PLEKHM2. Interacts with ECM29. |
| 亚细胞定位 | Cytoplasm, cytoskeleton. Note=Uniformly distributed between soma and neurites in hippocampal neurons. |
| 相似性 | Belongs to the kinesin-like protein family. Kinesin subfamily. Contains 1 kinesin-motor domain. |
| 功能 | Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Structural proteins in Arabidopsis provide cellular stability, facilitate protein transportation within the cell and are essential for cell growth. Three types of molecular motors are involved in the organization, dynamics and transport processes associated with the cytoskeleton (1). They include myosin, which transports cargo along actin filaments, and kinesin and dynein, which transport cargo along microtubules (1,2). These proteins regulate many cellular functions, including cell division and expansion, cell-to-cell communication, membrane trafficking and morphogenesis (2). In addition, the dynamin-like proteins are GTP-binding proteins involved in vesicle trafficking (3). Extensin is a structural protein that may be involved in cell wall assembly, while expansin is a cell wall loosening protein that induces stress relaxation and extension of cell walls and may control organ size, morphology and abscission (4,5). Also, xyloglucan (XG), a major hemicellulose in plants, is modified by the fucosyltransferase, XG FTase, which adds a terminal fucosyl residue to XG (6). |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
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文献和实验相关实验
Steady State ATPase Assays Coupled Enzyme System
from Rabbit Muscle in 50% glycerol) Procedure 1. Assemble microtubules for assays. E.g., 58 µL of 50 µM MTs = 50 µL 5.8 mg/mL tubulin + 0.5 µL 100 mM Mg・GTP, incubate for 30-60 min at 37°C
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
. Retrieved 2010-11-29. ^ Nielsen BS, Jørgensen S, Fog JU, Søkilde R, Christensen IJ, Hansen U, Brünner N, Baker A, Møller S, Nielsen HJ (October 2010). "High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival
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Kinesin/KIF5B Rabbit pAb(bs-11026R)-50ul/100ul/200ul
￥1180 - 2800
