FOSL1 | FRA1 (p-S265) Rabbit pAb(bs-3158R)-50ul/100ul/200ul产品图

FOSL1 | FRA1 (p-S265) Rabbit p

Ab(bs-3158R)-50ul/100ul/200ul
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  • ¥1180 - 2800
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  • bs-3158R
  • 2026年10月14日
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    • 规格:

      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-3158R
    英文名称phospho-FRA1 (Ser265) Rabbit pAb
    中文名称磷酸化FRA-1蛋白抗体
    英文别名FOSL1; Fos related antigen 1; FOS L1; FOS like antigen 1; FOSL 1; FOSL1 protein; FRA 1; Fos-related antigen 1; FOSL1_HUMAN; FRA-1.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Dog, Pig, Cow, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human FRA1 around the phosphorylation site of Ser265
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量28 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > Leucine Zipper

    Epigenetics and Nuclear Signaling > Transcription > Transcription Factors

    亚基Heterodimer.
    亚细胞定位Nucleus.
    相似性Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: mouse embryo tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-Phospho-FRA1(Ser265) Polyclonal Antibody, Unconjugated(bs-3158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片2
    Sample:
    _x000D_ Hela(Human) Cell Lysate at 30 ug
    _x000D_ U87MG(Human) Cell Lysate at 30 ug
    _x000D_ Primary: Anti- Phospho-FRA1 (Ser265) (bs-3158R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 28 kD
    _x000D_ Observed band size: 28 kD
    _x000D_
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FRA1 (Ser265)) Polyclonal Antibody, Unconjugated (bs-3158R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_ _x000D_

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    FOSL1 | FRA1 (p-S265) Rabbit pAb(bs-3158R)-50ul/100ul/200ul
    ¥1180 - 2800