Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gastrokine-1 Polyclonal Antibody, Unconjugated(bs-14441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- 详细信息
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- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-14441R |
| 英文名称 | Gastrokine 1 Rabbit pAb |
| 中文名称 | 胃窦粘膜蛋白GKN1抗体 |
| 英文别名 | 18 kDa antrum mucosa protein; AMP 18; AMP-18; AMP18; Antrum mucosa protein, 18-KD; BRICD 1; BRICD1; BRICHOS domain containing 1; CA 11; CA11; CA11 gene; CA11 protein; FOV; Foveolin; Gastrokine-1; Gastrokine1; GKN 1; Gkn1; GKN1_HUMAN; MGC70354; Protein CA11; UNQ489/PRO1005. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Gastrokine 1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 18 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Cell Type Markers > Epi / Endo-thelial |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Expressed in stomach. No expression is detected in cancer tissue or gastric cancer cell lines. |
| 相似性 | Belongs to the gastrokine family. Contains 1 BRICHOS domain. |
| 功能 | Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |


Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gastrokine-1 Polyclonal Antibody, Unconjugated(bs-14441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gastrokine-1 Polyclonal Antibody, Unconjugated(bs-14441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Gastrokine 1 Rabbit pAb(bs-14441R)-50ul/100ul/200ul
¥1180 - 2800








