Tissue/cell: rat skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COL6A5/Collagen VI alpha 5 Polyclonal Antibody, Unconjugated(bs-11047R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-11047R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11047R |
| 英文名称 | COL6A5 Rabbit pAb |
| 中文名称 | 6型胶原蛋白α5抗体 |
| 英文别名 | Collagen VI alpha 5; CO6A5_HUMAN; COL29A1; Col6a5; Collagen alpha-1(XXIX) chain; Collagen alpha-5(VI) chain; Collagen, type VI, alpha 5; Collagen, type XXIX, alpha-1; Gm7455; von Willebrand factor A domain-containing protein 4; VWA4. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human COL6A5/Collagen VI alpha 5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 288 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Trimers composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI) |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix. Note=Deposed in the extracellular matrix of skeletal muscle. |
| 组织特异性 | Expressed in skin, followed by lung, small intestine, colon and testis. In skin, it is expressed in the epidermis with strongest staining in suprabasal viable layers. In ATOD patients, it is absent in the most differentiated upper spinous and granular layers (at protein level). |
| 翻译后修饰 | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains |
| 相似性 | Belongs to the type VI collagen family.
Contains 10 VWFA domains. |
| 功能 | Collagen VI acts as a cell-binding protein. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a collagen that contains N- and C-terminal von Willebrand factor A-like domains. The encoded protein may interact with the alpha 1 and alpha 2 chains of collagen VI to form the complete collagen VI trimer. Sequence polymorphisms in this gene have been linked to atopic dermatitis (eczema). Two transcript variants, one protein-coding and the other probably not protein-coding, have been found for this gene. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 8.8 }}] {Jiajun Chen. et al. Integrating UHPLC-MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism. J PHARM ANAL. 2023 Aug;:} {WB} {Rat}
[IF={{ 8.469 }}] {Wu, Xiaosheng. et al. USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3/COL6A5 stabilisation. Cell Death Dis. 2021 Dec;13(1):1-11} {WB,IHC,IP} {Mouse,Human}
[IF={{ 5.714 }}] {Xianyan Liu. et al. Collagen VI antibody reduces atherosclerosis by activating monocyte/macrophage polarization in ApoE−/− mice. INT IMMUNOPHARMACOL. 2022 Oct;111:109100} {ELISA} {Mouse}
[IF={{ 5.2 }}] {Baoling Bai. et al. Pathological mechanisms of type 1 diabetes in children: investigation of the exosomal protein expression profile. FRONT ENDOCRINOL. 2023; 14: 1271929} {WB} {Human}
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COL6A5 Rabbit pAb(bs-11047R)-50ul/100ul/200ul
￥1180 - 2800
