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- bs-10140R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10140R |
| 英文名称 | PDE4A Rabbit pAb |
| 中文名称 | 磷酸二酯酶4A抗体 |
| 英文别名 | 5''-cyclic phosphodiesterase 4A; cAMP specific 3 5 cyclic phosphodiesterase 4A; cAMP specific phosphodiesterase; cAMP-specific 3''; cAMP-specific 3', 5'-cyclic phosphodiesterase 4A; DPDE2; dunce like phosphodiesterase E2; PDE 4A; PDE4; PDE46; PDE4A; PDE4A_HUMAN; PDE4A11; phosphodiesterase 4A; phosphodiesterase 4A cAMP specific; phosphodiesterase 4A, cAMP-specific(dunce; phosphodiesterase E2 dunce homolog; phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4; phosphodiesterase type 4A11. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PDE4A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 98 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Second Messenger > Nucleotide Messenger > cAMP |
| 亚基 | Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2. |
| 亚细胞定位 | Isoform 1: Cytoplasm, perinuclear region.
Isoform 2: Cytoplasm, perinuclear region. Cell projection, ruffle membrane. Isoform 4: Membrane; Peripheral membrane protein. Note=Isoform 4 has propensity for association with membranes. Isoform 6: Cytoplasm, perinuclear region. Isoform 7: Cytoplasm. Membrane. Note=Predominantly cytoplasmic. |
| 组织特异性 | Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level). |
| 翻译后修饰 | Phosphorylation by MAPKAPK2 its activation through PKA phosphorylation. Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
Proteolytically cleaved by caspase-3. |
| 相似性 | Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. |
| 功能 | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling. There are 5 isoforms. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDE4A) Polyclonal Antibody, Unconjugated (bs-10140R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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文献和实验相关实验
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GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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PDE4A Rabbit pAb(bs-10140R)-50ul/100ul/200ul
￥1180 - 2800
