LGMN | Legumain Rabbit pAb(bs-3907R)-50ul/100ul/200ul产品图
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LGMN | Legumain Rabbit pAb(bs-

3907R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-3907R
  • 2026年10月14日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-3907R
    英文名称Legumain Rabbit pAb
    中文名称半胱氨酸蛋白酶抗体
    英文别名AEP; Asparaginyl endopeptidase; EC 3.4.22.34; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1(legumain); PRSC1; LGMN_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Dog, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Legumain
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量46 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Hormone biosynthesis

    Cell Biology > Other Antibodies > Plant related targets

    Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases

    Immunology > Cell Type Markers > Non-CD > APC

    Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis

    Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Bone

    亚细胞定位Lysosome (By similarity).
    组织特异性Ubiquitous. Particularly abundant in kidney, heart and placenta.
    翻译后修饰Glycosylated.
    相似性Belongs to the peptidase C13 family.
    功能Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{3ug/Test}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Legumain) Polyclonal Antibody, Unconjugated (bs-3907R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    Sample: Kidney (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti- Legumain (bs-3907R) at 1/300 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 46 kD
    _x000D_ Observed band size: 55 kD
    _x000D_
    产品细节图片3
    Sample: Placenta (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti-Legumain (bs-3907R) at 1/300 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 46 kD
    _x000D_ Observed band size: 55 kD
    _x000D_
    产品细节图片4
    Blank control: HL60. _x000D_ Primary Antibody (green line): Rabbit Anti-Legumain antibody (bs-3907R) _x000D_ Dilution: 3μg /10^6 cells; _x000D_ Isotype Control Antibody (orange line): Rabbit IgG ._x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE_x000D_ Dilution: 1μg /test. _x000D_ Protocol_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    产品细节图片5
    Sample:
    _x000D_ Lane 1: Human JAR cell lysates
    _x000D_ Lane 2: Human MCF-7 cell lysates
    _x000D_ Primary: Anti-Legumain (bs-3907R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 46 kDa
    _x000D_ Observed band size: 55 kDa
    _x000D_ _x000D_

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    LGMN | Legumain Rabbit pAb(bs-3907R)-50ul/100ul/200ul
    ￥1180 - 2800