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Legumain Rabbit pAb(bs-3907R)-

50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-3907R
更新日期2025年10月24日

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应用范围：
产品信息以Bioss网站为准
规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-3907R
英文名称	Legumain Rabbit pAb
中文名称	半胱氨酸蛋白酶抗体
英文别名	AEP; Asparaginyl endopeptidase; EC 3.4.22.34; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1(legumain); PRSC1; LGMN_HUMAN.
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human, Mouse (Rat, Dog, Cow, Horse)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human Legumain
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	46 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Cancer Metabolism > Metabolic signaling pathway > Hormone biosynthesis Cell Biology > Other Antibodies > Plant related targets Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases Immunology > Cell Type Markers > Non-CD > APC Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Bone
亚细胞定位	Lysosome (By similarity).
组织特异性	Ubiquitous. Particularly abundant in kidney, heart and placenta.
翻译后修饰	Glycosylated.
相似性	Belongs to the peptidase C13 family.
功能	Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008].

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{IF}	{1:100-500}
{Flow-Cyt}	{3ug/Test}

P‌‌araformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Legumain) Polyclonal Antibody, Unconjugated (bs-3907R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti- Legumain (bs-3907R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 55 kD

Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-Legumain (bs-3907R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 55 kD

Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Legumain antibody (bs-3907R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Sample:
Lane 1: Human JAR cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti-Legumain (bs-3907R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 55 kDa

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文献和实验

该产品被引用文献

[IF={{ 5.988 }}] {Liu Haoge. et al. Number 2 Feibi Recipe Ameliorates Pulmonary Fibrosis by Inducing Autophagy Through the GSK-3β/mTOR Pathway. FRONT PHARMACOL. 2022 Jul;0:2714} {IHC,WB} {Mouse}

[IF={{ 3.6 }}] {Severin K. Lustenberger. et al. Towards imaging the immune state of cancer by PET: Targeting legumain with 11C-labeled P1-Asn peptidomimetics carrying a cyano-warhead. NUCL MED BIOL. 2024 Nov;138-139:108951} {IF} {Mouse}

[IF={{ 2.65 }}] {Haoge Liu. et al. Calycosin Ameliorates Bleomycin-Induced Pulmonary Fibrosis via Suppressing Oxidative Stress, Apoptosis, and Enhancing Autophagy. EVID-BASED COMPL ALT. 2022 Oct 11;2022:9969729} {WB} {Mouse}

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