Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LRCH4 Polyclonal Antibody, Unconjugated(bs-9330R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-9330R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 技术资料
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9330R |
| 英文名称 | LRCH4/LRRN1 Rabbit pAb |
| 中文名称 | 富含亮氨酸重复家庭蛋白4抗体 |
| 英文别名 | Leucine rich repeat neuronal protein 4; Leucine rich repeats and calponin homology(CH) domain containing 4; Leucine-rich neuronal protein; LRN; LRRN1; LRRN4; PP14183; SAP25; Sin3 associated polypeptide, 25kDa; LRCH4_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LRCH4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 73 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Other Antibodies |
| 亚细胞定位 | Cytoplasm. |
| 相似性 | Contains 1 CH (calponin-homology) domain. Contains 9 LRR (leucine-rich) repeats. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Members of the leucine-rich repeat family include LRCH1, LRCH2, LRCH3 and LRCH4. All family members contain one calponin-homology domain and nine leucine-rich repeats. LRCH4 (leucine-rich repeats and calponin homology (CH) domain containing 4), also known as LRN, LRRN1, LRRN4 or SAP25, is a 683 amino acid protein that belongs to the leucine-rich repeat family. The carboxy-terminus of LRCH4 may act as a membrane anchor between cells, while the amino-terminus contains the leucine-rich domains, which is thought to be involved in ligand binding. The calponin homology (CH) domain is suggested to confer Actin binding to a variety of cytoskeletal and signaling molecules. The gene encoding LRCH4 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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LRCH4/LRRN1 Rabbit pAb(bs-9330R)-50ul/100ul/200ul
￥1180 - 2800
