Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-GRIK3 antibody (bs-12007R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 2μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-12007R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12007R |
| 英文名称 | GRIK3 Rabbit pAb |
| 中文名称 | 谷氨酸受体红藻氨酸离子3/谷氨酸受体7抗体 |
| 英文别名 | EAA5; Excitatory amino acid receptor 5; GLR 7; GLR7; GLU R7; GLUR 7; GluR 7a; GluR-7; GLUR7; GluR7a; Glutamate receptor 7; Glutamate receptor; Glutamate receptor ionotropic kainate 3; GRIK 3; GRIK3; GRIK3_HUMAN; ionotropic kainate 3. |
| 产品应用 | Flow-Cyt=2ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GRIK3/GLR7 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 100 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmission > Receptors / Channels > Ligand-Gated Ion Channels > AMPA / Kainate Neuroscience > Neurotransmission > Receptors / Channels > Ligand-Gated Ion Channels > More Channels Neuroscience > Neurotransmitter > Amino Acids > Glutamate |
| 亚基 | Homotetramer, and heterotetramer with either GRIK4 or GRIK5. Interacts with PRKCABP. Interacts with NETO2. |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
| 相似性 | Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK3 subfamily. |
| 功能 | Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. It is not certain if the subunit encoded by this gene is subject to RNA editing as the other 2 family members (GRIK1 and GRIK2). A Ser310Ala polymorphism has been associated with schizophrenia, and there are conflicting reports of its association with the pathogenesis of delirium tremens in alcoholics. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/test} |
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GRIK3 Rabbit pAb(bs-12007R)-50ul/100ul/200ul
￥1180 - 2800
