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phospho-GCN2 (Thr667) Rabbit p

Ab(bs-3156R)-50ul/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-3156R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-3156R
    英文名称phospho-GCN2 (Thr667) Rabbit pAb
    中文名称磷酸化蛋白激酶GCN2蛋白抗体
    英文别名GCN2(phospho T667); p-GCN2(phospho T667); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; MGCN2.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse, Rat (Human, Dog, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量181 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases

    组织特异性Widely expressed.
    翻译后修饰Autophosphorylated on threonine residues.
    相似性Belongs to the protein kinase superfamily.
    Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 protein kinase domains.
    Contains 1 RWD domain.
    功能Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (bs-3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (bs-3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    Sample:
    Hippocampus (Mouse) Lysate at 40 ug
    Cerebral cortex (Mouse) Lysate at 40 ug
    Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 181 kD
    Observed band size: 170 kD
    产品细节图片4
    Sample:
    Cerebrum (Mouse) Lysate at 40 ug
    Cerebrum (Rat) Lysate at 40 ug
    Hippocampus (Mouse) Lysate at 40 ug
    Cerebral cortex (Mouse) Lysate at 40 ug
    Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 181 kD
    Observed band size: 170/181 kD

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    phospho-GCN2 (Thr667) Rabbit pAb(bs-3156R)-50ul/100ul/200ul
    ¥1180 - 2800