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产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-3156R |
| 英文名称 | phospho-GCN2 (Thr667) Rabbit pAb |
| 中文名称 | 磷酸化蛋白激酶GCN2蛋白抗体 |
| 英文别名 | GCN2(phospho T667); p-GCN2(phospho T667); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; MGCN2. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 181 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 组织特异性 | Widely expressed. |
| 翻译后修饰 | Autophosphorylated on threonine residues. |
| 相似性 | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 protein kinase domains. Contains 1 RWD domain. |
| 功能 | Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |



Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 181 kD
Observed band size: 170 kD

Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 181 kD
Observed band size: 170/181 kD
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antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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