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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3729R |
| 英文名称 | phospho-PKC gamma (Thr514) Rabbit pAb |
| 中文名称 | 磷酸化蛋白激酶C亚型G抗体 |
| 英文别名 | PKC gamma(phospho T514); p-PKC gamma(phospho T514); PKC gamma(phospho Thr514); PKC gamma(phospho T514); p-PKC gamma(Thr514); p-PKC gamma(Thr514); PKCC; PKCG; PRKCG; Protein kinase C gamma; Protein kinase C gamma polypeptide; Protein kinase C gamma type; SCA 14; SCA14; KPCG_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Chicken, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human PKC gamma around the phosphorylation site of Thr514 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 78 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Interacts with GRIA4. Interacts with CDCP1. Interacts with TP53INP1 and p53/TP53. |
| 亚细胞定位 | Cytoplasm.Cell membrane. |
| 组织特异性 | Expressed in Purkinje cells of the cerebellar cortex. |
| 翻译后修饰 | Autophosphorylation on Thr-674 appears to regulate motor functions of junctophilins, JPH3 and JPH4.Ubiquitinated. |
| 相似性 | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. |
| 功能 | This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验[IF={{ 4.586 }}] {Sanna MD et al. μ Opioid Receptor-Triggered Notch-1 Activation Contributes to Morphine Tolerance: Role of Neuron–Glia Communication. Mol Neurobiol. 2019 Jul 25.} {IF,WB} {Mouse}
[IF={{ 3.246 }}] {Xinyi He. et al. Glaesserella parasuis induces IL-17 production might through PKC-ERK/MAPK and IκB/NF-κB signaling pathways. VET MICROBIOL. 2022 Oct;273:109521} {WB} {Pig}
提示我模型中的通路究竟涉及哪个亚型，而我又没有那么多经费一个一个亚型去试，故我想能不能就检测总的PKC激活水平，毕竟有亚型被激活，那总的活化PKC水平也应该会升高啊！虽然敏感性和特异性肯定没有单独检测一个亚型的高。 还想请教：Phospho-PKC (pan) (gamma Thr514) Antibody 和Phospho-PKC (pan) (βII Ser660) Antibody 这两个抗体的针对的亚型有什么不同？既然都是pan，那是否说都是针对所有的亚型，只是磷酸化的残基不同
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer
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