Tissue/cell: Human kidney; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PHD2 Polyclonal Antibody, Unconjugated(bs-3686R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3686R |
| 英文名称 | PHD2 Rabbit pAb |
| 中文名称 | 脯氨酸羟化酶2抗体 |
| 英文别名 | SM 20; Zinc finger MYND domain containing protein 6; C1ORF12; Chromosome 1 Open Reading Frame 12; ECYT 3; ECYT3; EGL 9 homolog of C. elegans 1; EGL nine(C.elegans) homolog 1; Egl nine homolog 1; Egl nine like protein 1; EGLN 1; EGLN1; HIF PH2; HIF Prolyl Hydroxylase 2; HIFP4H 2; HIFPH2; HPH 2; HPH2; Hypoxia inducible factor prolyl hydroxylase 2; ORF13; P4H2; PHD 2; PHD2; PNAS 118; PNAS 137; Prolyl Hydroxylase Domain Containing Protein 2; Rat Homolog of SM20; SM20; ZMYND6. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PHD2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 47 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cancer > Invasion/microenvironment > Hypoxia > Hydroxylases Cardiovascular > Hypoxia > Prolyl Hydroxylase Epigenetics and Nuclear Signaling > Cardiovascular/Immune > Hypoxia > Prolyl hydroxylase Metabolism > Pathways and Processes > Metabolism processes > Hypoxia |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 组织特异性 | According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle. |
| 相似性 | Contains 1 Fe2OG dioxygenase domain. Contains 1 MYND-type zinc finger. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | PHD2 protein catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. In the presence of oxygen, PHD2 converts specific prolyl residues in HIF alpha to hydroxyproline, leading to HIF alpha proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF levels are stable. This results in increased angiogenesis as HIF1 alpha regulates the expression of many angiogenesis-related genes. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-PHD2 (bs-3686R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 43 kD
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-PHD2 (bs-3686R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 43 kD
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PHD2) polyclonal Antibody, Unconjugated (bs-3686R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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PHD2 Rabbit pAb(bs-3686R)-50ul/100ul/200ul
￥1180 - 2800
