Sample:
_x000D_
MCF-7(Human) Cell Lysate at 40 ug
_x000D_
A431(Human) Cell Lysate at 40 ug
_x000D_
U2os(Human) Cell Lysate at 40 ug
_x000D_
Primary: Anti-ZNF185 (bs-6119R) at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 76/39 kD
_x000D_
Observed band size: 67 kD
- ￥1180 - 2800
- Bioss
- bs-6119R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6119R |
| 英文名称 | ZNF185 Rabbit pAb |
| 中文名称 | 锌指蛋白185抗体 |
| 英文别名 | LIM domain protein ZNF185; P1 A; Zinc finger protein 185(LIM domain); ZN185_HUMAN. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ZNF185 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 76(human)/39(mo, kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Cytoplasm, cytoskeleton. Cell junction, focal adhesion. |
| 组织特异性 | Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood. |
| 相似性 | Contains 1 LIM zinc-binding domain. |
| 功能 | May be involved in the regulation of cellular proliferation and/or differentiation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | ZNF185 encodes a LIM domain zinc finger protein. It may be involved in the regulation of cellular proliferation and/or differentiation. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {3ug/Test} |
Blank control: A431.
_x000D_ Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-6199R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-6199R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
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ZNF185 Rabbit pAb(bs-6119R)-50ul/100ul/200ul
￥1180 - 2800
