Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (bs-2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
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- bs-2646R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2646R |
| 英文名称 | LAG3 Rabbit pAb |
| 中文名称 | 淋巴细胞活化基因-3（CD223）抗体 |
| 英文别名 | Lymphocyte activation gene 3 protein; Lymphocyte activation gene 3; CD223; FDC protein; LAG 3; LAG-3; LAG3_HUMAN. |
| 产品应用 | Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LAG-3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 58 kDa |
| 实际分子量 | 70 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Adaptive Immunity > T Cells > CD Immunology > Cell Type Markers > CD > NK Cells Immunology > Secreted Molecules > Other secreted molecules |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | On cell surface of activated NK and T-lymphocytes. |
| 相似性 | Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | Involved in lymphocyte activation. Binds to HLA class-II antigens. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg /test} |
Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (bs-2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (bs-2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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[IF={{ 5.9 }}] {Rui Guo. et al. A novel bispecific aptamer targeting LAG3 and HER2 enhances T cell-mediated immunotherapy against HER2-positive cancer cells. FRONT IMMUNOL. 2025 Jul;16:} {} {Human}
[IF={{ 2.976 }}] {Chen Y et al. Antitumor effects of the silencing of programmed cell death ligand 1 in colorectal cancer via immunoregulation. (2018) Oncol.Rep.} {IHC} {Mouse}
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LAG3 Rabbit pAb(bs-2646R)-50ul/100ul/200ul
￥1180 - 2800
