Sample:
Liyer(Mouse) Cell Lysate at 40 ug
Primary: Anti-Tartrate Resistant Acid Phosphatase (bs-6434R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
- ￥1180 - 2800
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- bs-6434R
- 2025年10月16日
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- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6434R |
| 英文名称 | ACP5 Rabbit pAb |
| 中文名称 | 抗酒石酸酸性磷酸酶5型/5型酸性磷酸酶抗体 |
| 英文别名 | Type 5 acid phosphatase; Acid phosphatase 5 tartrate resistant; PPA5_HUMAN; T5ap; Tartrate resistant acid ATPase; Tartrate resistant acid phosphatase type 5; Tartrate resistant acid phosphatase type 5 precursor; Tartrate-resistant acid ATPase; Tartrate-resistant acid phosphatase type 5; TR AP; TR-AP; TrATPase; Type 5 acid phosphatase. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, A431 (Rat, Dog, Pig, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human TRAP/Tartrate Resistant Acid Phosphatase |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 34 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers > Enzymes > Phosphatases Cell Biology > Cell Cycle > Kinases/Phosphatases > Phosphatases Kits/ Lysates/ Other > Kits > Cell Metabolism Kits > Other Metabolism Assay |
| 亚基 | Exists either as monomer or, after proteolytic processing, as a dimer of two chains linked by disulfide bond(s). |
| 亚细胞定位 | Lysosome. |
| 相似性 | Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. |
| 功能 | Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI) [MIM:607944]. A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. Note=ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti-Tartrate Resistant Acid Phosphatase (bs-6434R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 35 kD
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti-Tartrate Resistant Acid Phosphatase (bs-6434R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 35 kD
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-ACP5 antibody (bs-6434R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-ACP5 antibody (bs-6434R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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[IF={{ 2.5 }}] {Adem Tokpinar. et al. The effect of ionizing radiation on the fetal bone development in pregnant rats: Role of melatonin. MICROSC RES TECHNIQ. 2023 Sep;:} {IHC} {Rat}
[IF={{ 0 }}] {Sun, Weijia, et al. "Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity." Cell Discovery 2 (2016).} {WB} {="Mouse"}
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ACP5 Rabbit pAb(bs-6434R)-50ul/100ul/200ul
￥1180 - 2800
