Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SCD1 Polyclonal Antibody, Unconjugated(bs-3787R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-3787R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3787R |
| 英文名称 | SCD Rabbit pAb |
| 中文名称 | 固醇酰辅酶A脱氢酶1抗体 |
| 英文别名 | SCD_HUMAN; Stearoyl-CoA desaturase; EC:1.14.19.1; hSCD1; Acyl-CoA desaturase; delta(9)-desaturase(delta-9 desaturase); Fatty acid desaturase; FADS5; SCD1; SCDOS; MSTP008; |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SCD |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 41 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Fatty acids Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation Metabolism > Types of disease > Obesity Signal Transduction > Metabolism > Lipid metabolism |
| 亚细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein (Probable). |
| 相似性 | Belongs to the fatty acid desaturase family. |
| 功能 | Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SCD1) polyclonal Antibody, Unconjugated (bs-3787R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 9.8 }}] {Yanghuan Yu. et al. MiRNA-seq and mRNA-seq revealed the mechanism of fluoride-induced cauda epididymal injury. SCI TOTAL ENVIRON. 2024 Jun;930:172895} {WB} {Mouse}
[IF={{ 9.685 }}] {Wang, Nan. et al. USP7- and PRMT5-dependent G3BP2 stabilization drives de novo lipogenesis and tumorigenesis of HNSC. CELL DEATH DIS. 2023 Mar;14(3):1-13} {WB} {Human}
[IF={{ 6.3 }}] {Jing Zhang. et al. Dietary supplementation of sodium butyrate enhances lactation performance by promoting nutrient digestion and mammary gland development in dairy cows. ANIM NUTR. 2023 Sep;:} {WB} {Bovine}
[IF={{ 6.1 }}] {Jiangtao Huang. et al. Enhancement of PPARα-Inhibited Leucine Metabolism-Stimulated β-Casein Synthesis and Fatty Acid Synthesis in Primary Bovine Mammary Epithelial Cells. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXX} {WB} {Bovine}
[IF={{ 5.6 }}] {Yating Fan. et al. β-Sitosterol Suppresses Lipopolysaccharide-Induced Inflammation and Lipogenesis Disorder in Bovine Mammary Epithelial Cells. INT J MOL SCI. 2023 Jan;24(19):14644} {WB} {Bovine}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
进口分装 F0252 血清淀粉样蛋白(SAA) ELISA 48T/96T 2000/3000 进口分装 F0253 可溶性细胞表面分化抗原40 配体 (sCD40L) ELISA 48T/96T 2000/3000 进口分装 F0254 ★ 干细胞因子(SCF) ELISA 48T/96T 1200/2000 进口分装 F0255 干细胞因子受体(SCF R) ELISA 48T/96T 2000/3000 进口分装 F
进口分装 F0252 血清淀粉样蛋白(SAA) ELISA 48T/96T 2000/3000 进口分装 F0253 可溶性细胞表面分化抗原40 配体 (sCD40L) ELISA 48T/96T 2000/3000 进口分装 F0254 ★ 干细胞因子(SCF) ELISA 48T/96T 1200/2000 进口分装 F0255 干细胞因子受体(SCF R) ELISA 48T/96T 2000/3000 进口分装 F
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SCD Rabbit pAb(bs-3787R)-50ul/100ul/200ul
￥1180 - 2800
